Early in the spring of 1900, during the occupation of Cuba by the United States, a commission appointed by Surgeon-General Sternberg (himself one of the most energetic students of the disease) undertook fresh investigations.
Dr.Walter Reed, Professor of Bacteriology in the Army Medical School, was placed in charge: Dr.Carroll of the United States Army, Dr.
Agramonte of Havana and Dr.Jesse W.Lazear were the other members.

At the Johns Hopkins Hospital, we were deeply interested in the work, as Dr.Walter Reed was a favorite pupil of Professor Welch, a warm friend of all of us, and a frequent visitor to our laboratories.

Dr.Jesse Lazear, who had been my house physician, had worked with Dr.Thayer and myself at malaria, and gave up the charge of my clinical laboratory to join the commission.
(5) Matthew Carey: A Short Account of the Malignant Fever, Philadelphia, 1793.
(6) R.La Roche: Yellow Fever, 2 vols., Philadelphia, 1855.
Many scientific discoveries have afforded brilliant illustrations of method in research, but in the work of these men one is at a loss to know which to admire more--the remarkable accuracy and precision of the experiments, or the heroism of the men--officers and rank and file of the United States Army; they knew all the time that they were playing with death, and some of them had to pay the penalty! The demonstration was successful--beyond peradventure--that yellow fever could be transmitted by mosquitoes, and equally the negative proposition--that it could not be transmitted by fomites.

An interval of twelve or more days was found to be necessary after the mosquito has bitten a yellow fever patient before it is capable of transmitting the infection.

Lazear permitted himself to be bitten by a stray mosquito while conducting his experiments in the yellow fever hospital.