By the Treaty of Cambray Francis I.relinquished his claims on Italy and abandoned his Italian supporters without conditions, receiving in exchange the possession of Burgundy.

The French allies who were sacrificed on this occasion by the Most Christian to the Most Catholic Monarch consisted of the Republics of Venice and Florence, the Dukes of Milan and Ferrara, the princely Houses of Orsini and Fregosi in Rome and Genoa, together with the Angevine nobles in the realm of Naples.

The Paix des Dames, as this act of capitulation was called (since it had been drawn up in private conclave by Louise of Savoy and Margaret of Austria, the mother and the aunt of the two signatories), was a virtual acknowledgment of the fact that French influence in Italy was at an end.[1] The surrender of Italy by Francis made it necessary that Charles V.
should put in order the vast estates to which he now succeeded as sole master.

He was, moreover, Emperor Elect; and he judged this occasion good for assuming the two crowns according to antique custom.
Accordingly in July, 1529, he caused Andrea Doria to meet him at Barcelona, crossed the Mediterranean in a rough passage of fourteen days, landed at Genoa on August 12, and proceeded by Piacenza, Parma and Modena to Bologna, where Clement VII.

was already awaiting him.